FlowCytomix™ Simplex Kits

Simplex Kits contain reagents for the detection of one specific analyte.

For measurement of multiple analytes, a combination of almost all Simplex Kits is possible.

Reagents provided

Fluorescent Beads conjugated to capture antibodies
Biotin- or PE- conjugated detection antibody
Reference Standard Protein

Basic Kits include essential core reagents and are required to successfully perform assays using Simplex Kits:

For mouse and rat Simplex Kits the mouse/rat Basic Kit is required.

For human Simplex Kits PE (E-selectin, ICAM-1, ICAM-3, PECAM-1) the human Basic Kit PE (BMS8421FF) is required. In case Simplex Kits and Simplex Kits PE are combined the the human Basic Kit (BMS8420FF) is required.

For all other human Simplex Kits the human Basic Kit is required.

Analyte	Species	Cat. Nr.	Standard Range	Sensitivity	Sample Volume
Adiponectin	Human	BMS82032FF	0.1 - 50 ng/ml	0.06 ng/ml	25 µl (1:250 prediluted)
APO-1/Fas	Human	BMS80245FF	34 - 25,000 pg/ml	10.0 pg/ml	25 µl
Caspase-3	Human	BMS82012FF	82 - 60,000 pg/ml	20 pg/ml	25 µl
Caspase-9	Human	BMS82025FF	0.2 - 150 ng/ml	0.07 ng/ml	25 µl
CD30	Human	BMS8240FF	0.3 - 250 ng/ml	5.1 pg/ml	25 µl
CD40L	Human	BMS8239/2FF	55 - 40,000 pg/ml	23.4 pg/ml	25 µl
CD44var (v6)	Human	BMS80210FF	137 - 100,000 pg/ml	126.0 pg/ml	25 µl (1:30 prediluted)
CRP	Human	BMS8288FF	0.1 - 70 ng/ml	0.07 ng/ml	25 µl (1:250 prediluted)
CXCL1/KC	Mouse	BMS86019FF	4.8 - 3,500 pg/ml	3.0 pg/ml	25 µl
E-selectin	Human	BMS8205FF	4.0 - 3,000 ng/ml	1.2 ng/ml	25 µl
EGF	Human	BMS82070FF	27 - 20,000 pg/ml	22.7 pg/ml	25 µl
G-CSF	Human	BMS82001FF	34 - 25,000 pg/ml	3.4 pg/ml	25 µl
GM-CSF	Mouse	BMS8612FF	27 - 20,000 pg/ml	10.9 pg/ml	25 µl
GM-CSF	Rat	BMS8633FF	27 - 20,000 pg/ml	5.0 pg/ml	25 µl
HGF	Human	BMS82069FF	206 - 150.000 pg/ml	52 pg/ml	25 µl
ICAM-1	Human	BMS80201FF	5.4 - 4,000 ng/ml	5.3 ng/ml	25 µl
ICAM-3	Human	BMS8218FF	11 - 8,000 ng/ml	4.8 ng/ml	25 µl
IFN-alpha	Human	BMS8216FF	27 - 20,000 pg/ml	8.06 pg/ml	25 µl
IFN-gamma	Human	BMS8228FF	27 - 20,000 pg/ml	1.6 pg/ml	25 µl
IFN-gamma	Rat	BMS8621FF	27 - 20,000 pg/ml	8.3 pg/ml	25 µl
IFN-gamma	Mouse	BMS8606/2FF	27 - 20,000 pg/ml	6.5 pg/ml	25 µl
IL-10	Human	BMS8215FF	27 - 20,000 pg/ml	1.9 pg/ml	25 µl
IL-10	Mouse	BMS8614/2FF	27 - 20,000 pg/ml	5.4 pg/ml	25 µl
IL-12 (p70)	Human	BMS8238FF	27 - 20,000 pg/ml	1.5 pg/ml	25 µl
IL-12 (p70)	Mouse	BMS86004FF	27 - 20,000 pg/ml	8.6 pg/ml	25 µl
IL-13	Human	BMS8231FF	27 - 20,000 pg/ml	4.5 pg/ml	25 µl
IL-13	Mouse	BMS86015FF	27 - 20,000 pg/ml	9.3 pg/ml	25 µl
IL-17	Mouse	BMS86001FF	27 - 20,000 pg/ml	2.4 pg/ml	25 µl
IL-17A	Human	BMS82017FF	13.7 - 10,000 pg/ml	2.5 pg/ml	25 µl
IL-18	Human	BMS8267FF	27 - 20,000 pg/ml	3.34 pg/ml	25 µl
IL-18	Mouse	BMS8618FF	412 - 300,000 pg/ml	116.9 pg/ml	25 µl
IL-1alpha	Human	BMS80243FF	1.4 - 1,000 pg/ml	0.5 pg/ml	25 µl
IL-1alpha	Mouse	BMS8611FF	27 - 20,000 pg/ml	15.7 pg/ml	25 µl
IL-1alpha	Rat	BMS8627FF	27 - 20,000 pg/ml	8.5 pg/ml	25 µl
IL-1beta	Mouse	BMS86002FF	69 - 50.000 pg/ml	34.3 pg/ml	25 µl
IL-1beta	Human	BMS8224FF	27 - 20,000 pg/ml	4.2 pg/ml	25 µl
IL-2	Mouse	BMS8601FF	27 - 20,000 pg/ml	8.8 pg/ml	25 µl
IL-2	Human	BMS8221FF	27 - 20,000 pg/ml	16.4 pg/ml	25 µl
IL-22	Human	BMS82047FF	110 - 80,000 pg/ml	43.3 pg/ml	25 µl
IL-22	Mouse	BMS86016FF	6.9 - 5,000 pg/ml	5.5 pg/ml	25 µl
IL-23	Human	BMS82023FF	69 - 50,000 pg/ml	21.9 pg/ml	25 µl
IL-23	Mouse	BMS86017FF	55 - 40,000 pg/ml	14.5 pg/ml	25 µl
IL-4	Mouse	BMS8613FF	27 - 20,000 pg/ml	0.7 pg/ml	25 µl
IL-4	Human	BMS8225FF	27 - 20,000 pg/ml	20.8 pg/ml	25 µl
IL-4	Rat	BMS8628FF	3.0 - 2,000 pg/ml	0.3 pg/ml	25 µl
IL-5	Mouse	BMS8610FF	27 - 20,000 pg/ml	4.0 pg/ml	25 µl
IL-5	Human	BMS8278FF	27 - 20,000 pg/ml	1.6 pg/ml	25 µl
IL-6	Mouse	BMS8603FF	27 - 20,000 pg/ml	2.2 pg/ml	25 µl
IL-6	Human	BMS8213FF	27 - 20,000 pg/ml	1.2 pg/ml	25 µl
IL-8	Human	BMS8204FF	13.7 - 10,000 pg/ml	0.5 pg/ml	25 µl
IP-10	Human	BMS8284FF	17 - 12,500 pg/ml	6.0 pg/ml	25 µl
IP-10	Mouse	BMS86018FF	41 - 30,000 pg/ml	9.8 pg/ml	25 µl
Iso-Insulin	Human	BMS82003FF	274 - 200,000 pg/ml	80 pg/ml	25 µl
Leptin	Human	BMS82039/2FF	0.3 - 250 ng/ml	0.05 ng/ml	25 µl
MCP-1	Mouse	BMS86005FF	55 - 40,000 pg/ml	42.0 pg/ml	25 µl
MCP-1	Human	BMS8281FF	41 - 30,000 pg/ml	2.2 pg/ml	25 µl
MCP-1	Rat	BMS8631FF	27 - 20,000 pg/ml	0.5 pg/ml	25 µl
MCP-3	Mouse	BMS86006FF	27 - 20,000 pg/ml	1.4 pg/ml	25 µl
MIG	Human	BMS8285FF	6.9 - 5,000 pg/ml	0.9 pg/ml	25 µl
MIP-1alpha	Mouse	BMS86013FF	27 - 20,000 pg/ml	1.8 pg/ml	25 µl
MIP-1alpha	Human	BMS82029FF	13.7 - 10,000 pg/ml	1.0 pg/ml	25 µl
MIP-1beta	Mouse	BMS86014FF	27 - 20,000 pg/ml	14.9 pg/ml	25 µl
MIP-1beta	Human	BMS82030FF	4.0 - 3,000 pg/ml	1.0 pg/ml	25 µl
MMP-13	Human	BMS82022FF	0.17 - 125 ng/ml	0.05 ng/ml	25 µl
MMP-9	Human	BMS82016FF	0.1 - 100 ng/ml	95 pg/ml	25 µl (1:50 prediluted)
MPO	Human	BMS82038FF	0.14 - 100 ng/ml	0.02 ng/ml	25 µl
NGF	Human	BMS82044FF	412 - 300,000 pg/ml	126.75 pg/ml	25 µl
OPG	Human	BMS82021FF	27 - 20,000 pg/ml	7.9 pg/ml	25 µl
P-selectin	Human	BMS8219/2FF	2.8 - 2,000 ng/ml	1.2 ng/ml	25 µl
PAI-1	Human	BMS82033FF	137 - 100,000 pg/ml	13.5 pg/ml	25 µl (1:10 prediluted)
PDGF-BB	Human	BMS82071FF	21 - 15.000 pg/ml	3.4 pg/ml	25 µl
PECAM-1	Human	BMS8229FF	4.0 - 3,000 ng/ml	0.8 ng/ml	25 µl
RANTES	Mouse	BMS86009FF	27 - 20,000 pg/ml	6.1 pg/ml	25 µl
RANTES	Human	BMS8287FF	41 - 30,000 pg/ml	25 pg/ml	25 µl (1:10 prediluted)
Resistin	Human	BMS82040FF	55 - 40,000 pg/ml	1.7 pg/ml	25 µl
t-PA	Human	BMS8258FF	27 - 20,000 pg/ml	4.8 pg/ml	25 µl
TGF-beta1	Human	BMS8249FF	13.7 - 10,000 pg/ml	10.0 pg/ml	25 µl (pretreated)
TGF-beta1	Mouse	BMS8608FF	13.7 - 10,000 pg/ml	10.0 pg/ml	25 µl (pretreated)
TIMP-1	Human	BMS82018FF	137 - 100,000 pg/ml	28 pg/ml	25 µl (1:50 prediluted)
TNF-alpha	Human	BMS8223FF	27 - 20,000 pg/ml	3.2 pg/ml	25 µl
TNF-alpha	Rat	BMS8622FF	27 - 20,000 pg/ml	4.3 pg/ml	25 µl
TNF-alpha	Mouse	BMS8607/2FF	27 - 20,000 pg/ml	2.1 pg/ml	25 µl
TNF-beta	Human	BMS8202FF	27 - 20,000 pg/ml	2.4 pg/ml	25 µl
TNF-R (60kDa)	Human	BMS8203FF	0.14 - 100 ng/ml	0.08 ng/ml	25 µl
VCAM-1	Human	BMS8232/2FF	3.0 - 2,000 ng/ml	0.9 ng/ml	25 µl (1:20 prediluted)
VEGF-A	Human	BMS80277FF	27 - 20,000 pg/ml	7.2 pg/ml	25 µl

Possible Combinations

Assay performance data presented was generated by Bender MedSystems, and is considered typical for a routine experiment in our laboratories (data on file at our Corporate Office in Vienna, Austria). Each laboratory using this product should establish its own performance characteristics, and these may vary from those presented on the website.