FlowCytomix™ Multiplex Kits

Multiplex Kits contain all required reagents for detection of multiple analytes in a single sample on most flow cytometers.

Reagents provided

Fluorescent Beads conjugated to capture antibodies
Biotin-conjugated detection antibody
PE-conjugated detection antibody
Reference Standard Protein
Streptavidin-PE, if required
Cytometer Setup Beads
Filter Plate, Plate Covers
Assay Buffer
Reagent Dilution Buffer, if required
The FlowCytomix™ Pro 2.3 (Cat. No. BMS8401FF) Software is complimentary and can be ordered at europe@ebioscience.com or downloaded at www.bendermedsystems.com/software-download

Analyte	Species	Cat. Nr.	Standard Range	Sensitivity	Sample Volume
Adhesion 6plex	Human	BMS812FF	sE-selectin: 4.0 - 3,000 ng/ml sICAM-1: 5.0 - 4,000 ng/ml sICAM-3: 11 - 8000 ng/ml sPECAM-1: 4.0 - 3,000 ng/ml sP-selectin: 19 - 14,000 ng/ml sVCAM-1: 5.0 - 4,000 ng/ml	sE-selectin: 1.2 ng/ml sICAM-1: 5.3 ng/ml sICAM-3: 4.8 ng/ml sPECAM-1: 0.8 ng/ml sP-selectin: 5.7 ng/ml sVCAM-1: 0.2 ng/ml	25 µl
Cardiovascular 6plex	Human	BMS811/2FF	CD40L: 55 - 40,000 pg/ml IL-6: 27 - 20,000 pg/ml IL-8: 13.7 - 10,000 pg/ml MCP-1: 41 - 30,000 pg/ml P-selectin: 3.0 - 2,000 ng/ml t-PA: 27 - 20,000 pg/ml	CD40L: 24.3 pg/ml IL-6: 0.4 pg/ml IL-8: 1.1 pg/ml MCP-1: 1.3 pg/ml P-selectin: 1.2 ng/ml t-PA: 4.8 pg/ml	25 µl
Chemokines 6plex	Mouse	BMS821FF	27 - 20,000 pg/ml MCP-1: 55 - 40,000 pg/ml	GM-CSF: 12.2 pg/ml MCP-1: 42 pg/ml MCP-3: 1.4 pg/ml MIP-1alpha: 1.8 pg/ml MIP-1beta: 14.9 pg/ml RANTES: 6.1 pg/ml	25 µl
Chemokines 6plex	Human	BMS813FF	G-CSF: 34.3 - 25,000 pg/ml IL-8: 13.7 - 10,000 pg/ml MCP-1: 41 - 30,000 pg/ml MIG: 6.9 - 5,000 pg/ml MIP-1alpha: 13.7 - 10,000 pg/ml MIP-1beta: 4.1 - 3,000 pg/ml	MIP-1alpha: 1.0 pg/ml IL-8: 2.2 pg/ml MIP-1beta: 1.0 pg/ml G-CSF: 3.4 pg/ml MIG: 0.9 pg/ml MCP-1: 2.2 pg/ml	25 µl
Cytokines 6plex	Rat	BMS825/2FF	13.7 - 10,000 pg/ml IL-4: 3.0 - 2,000 pg/ml	rGM-CSF: 5.0 pg/ml rIFN-gamma: 0.6 pg/ml rIL-1alpha: 8.5 pg/ml rIL-4: 0.3 pg/ml rMCP-1: 0.5 pg/ml rTNF-alpha: 4.3 pg/ml	25 µl
Obesity 9plex	Human	BMS816/2FF	sCD40L: 55 - 40,000 pg/ml sICAM-1: 5 - 4,000 ng/ml IL-6: 27 - 20,000 pg/ml Leptin: 0.3 - 250 ng/ml MCP-1: 41 - 30,000 pg/ml MPO: 1.4 - 1,000 ng/ml OPG: 27 - 20,000 pg/ml Resistin: 55 - 40,000 pg/ml sTNF-R (60kDa): 0.14 - 100 ng/ml	sCD40L: 12.3 pg/ml sICAM-1: 5.3 ng/ml IL-6: 4.4 pg/ml Leptin: 0.05 ng/ml MCP-1: 18.2 pg/ml MPO: 0.02 ng/ml OPG: 7.9 pg/ml Resistin: 1.7 pg/ml sTNF-R (60kDa): 0.08 ng/ml	25 µl
Th1/Th2 10plex	Mouse	BMS820FF	27 - 20,000 pg/ml	mGM-CSF: 10.9 pg/ml mIFN-gamma: 6.5 pg/ml mIL-1alpha: 15.7 pg/ml mIL-2: 8.8 pg/ml mIL-4: 0.7 pg/ml mIL-5: 4.0 pg/ml mIL-6: 2.2 pg/ml mIL-10: 5.4 pg/ml mIL-17: 2.4 pg/ml mTNF-alpha: 2.1 pg/ml	25 µl
Th1/Th2 11plex	Human	BMS810FF	27 - 20,000 pg/ml hIL-8: 14 - 10,000 pg/ml	hIL-1beta: 4.2 pg/ml IL-2: 16.4 pg/ml hIL-4: 20.8 pg/ml hIL-5: 1.6 pg/ml hIL-6: 1.2 pg/ml hIL-8: 0.5 pg/ml hIL-10: 1.9 pg/ml hIL-12p70: 1.5 pg/ml hTNF-alpha: 3.2 pg/ml hTNF-beta: 2.4 pg/ml hIFN-gamma: 1.6 pg/ml	25 µl

Assay performance data presented was generated by Bender MedSystems, and is considered typical for a routine experiment in our laboratories (data on file at our Corporate Office in Vienna, Austria). Each laboratory using this product should establish its own performance characteristics, and these may vary from those presented on the website.