Bender MedSystems High Sensitivity ELISA Technology

Conventional sandwich ELISA using a Biotin-Streptavidin-HRP reaction for detection.

Amplification reagent I contains biotinyl-tyramide. HRP converts multiple biotinyl-tyramide molecules into highly reactive derivates (free radicals). These free radicals bind covalently to any protein in the well (e.g. the blocking proteins).

The amount of reacted biotinyl-tyramide is proportional to the amount of HRP in the well. Following incubation, unbound biotinyl-tyramide is removed during a wash step. Amplification reagent II contains Streptavidin-HRP, which binds to the biotin sites, created during the biotinyl-tyramide reaction. This amplification step effectively multiplies the quantity of HRP molecules available at the surface for the substrate reaction.

High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.