Bender MedSystems ELISA Technology

The Enzyme-Linked ImmunoSorbent Assay, or ELISA, is a fast, accurate, and sensitive immunochemical assay used to measure the presence of an antibody or antigen in a sample. ELISAs typically use antibodies and/or antigens coupled to easily assayed enzymes as detection reagents. These enzyme ‘conjugates' act on chromogenic or fluorogenic substrates that ultimately produce an amplified detection signal. ELISA tests can routinely detect picogram (10^-12) concentrations of analytes in serum, plasma and cell culture supernatants.

Sandwich ELISA

(1) ELISA plate is coated with a capture antibody; (2) Sample is added, and the respective antigen present binds to capture antibody; (3) Biotin-conjugated secondary detection antibody is added, and binds to the antigen captured by the first antibody; (4) Streptavidin-HRP is added and binds to the biotin conjugated detection antibody; (5) Coloured product is formed in proportion to the amount of antigen present in the sample; The reaction is terminated by addition of acid and absorbance is measured at 450 nm;